Info-enriched characterization of cell populations. Morphological data from pictures can incorporate towards the richness of flow cytometry information. One example is, the determine displays an normally regular apoptosis assay utilizing Annexin V and PI, including cell imaging to characterize cells in Just about every populace to expose morphologically distinctive functions. These insights could not are already attained from multiplex staining by itself.
As samples are acquired on the Attune CytPix Flow Cytometer, the superior-pace brightfield digicam captures and merchants illustrations or photos of detected occasions in a charge approximately 6,000 images for every next, depending upon the flow level and graphic size. For increased flexibility, Attune Cytometric Software permits you to change the impression capture frequency as needed and select a certain gate for impression capture, if expected.
(B) Uncooked 264.seven macrophages in many cases are annotated inaccurately with regard to particle rely because of their complexity and noncircular borders. The volume of singlets accessible for Evaluation improves once the person trains and employs a far more exact model.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing by using a significant-speed brightfield digicam for simultaneous substantial throughput flow cytometry and significant resolution brightfield imaging.
*Pricing is indicative only and it is subject to change. Make sure you Call your Agilent representative with any issues. The information set forth herein will not be an offer or even a commitment, both of which can be subject to credit rating acceptance, stipulations, and availability with Agilent. Agilent Monetary Solutions is accessible in picked countries.
Moreover, it is illuminating to find out what number of doublets slip through regular singlet gates, and fantastic to validate the standing of unanticipated double-favourable events in my Investigation."
Optimizing gating system. Even strong handbook singlet gating is error-prone and stays a subjective conclusion position in Practically all flow cytometry assays. Imaging may be used to verify and modify gates to incorporate only single cells of curiosity.
Reliable graphic good quality even at substantial throughput Acoustic concentrating as well as a higher-speed digicam Incorporate to graphic these T cells consistently at small or higher flow rates. Conveniently regulate concentrate and digital camera options to meet experimental requirements.
Early signs of this contamination experienced to begin with been dismissed as debris. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also lets you use morphological features to discover appealing subpopulations that will not be apparent from flow cytometry knowledge by itself.
Users can watch function illustrations or photos in authentic-time to verify sample good quality, optimize protocols, and file the morphology of each and every mobile populace identified for Examination. Imaging capture also Added benefits from acoustic focusing, which helps to placement the cells to make sure that a pointy, centered graphic is obtained.
AI-pushed automated impression Investigation Using the Attune Cytometric Computer software interprets event functions into distinct morphology parameters that can be combined with normal fluorescence and scatter parameters to expose remarkably reproducible population changes and exclusive subsets.
Application Be aware: Automatic graphic Examination lessens person-to-consumer variability in flow cytometry gating tactics
Leverage the power of Highly developed technological know-how and innovation to accelerate your discoveries. Belief within the Attune Flow Cytometer family members to provide the reliability, speed, and precision that set your perform apart.
Versatile payment strategy from Agilent Financial Options assist you to get the vital instrumentation you would like for immediate job, or to develop your lab.
Gating on these extended parameters enables you to immediately and precisely identify populations of interest and ensure gating approach with little if any guide review. The Attune application features use of condition-of-the-art equipment Understanding Examination equipment in an expertly built person interface, more info making it extremely convenient to use.
Because the mobile line had Formerly been analyzed over the Attune CytPix Flow Cytometer, the researchers went again to the images and ended up ready to document the microbial infection at the very least 5 days before.
Agilent Value Promise assures you not less than 10 years of instrument use from your day you buy. In any other case, We're going to credit history you Together with the residual value of the procedure towards an upgraded design.
What appeared to be a homogeneous populace is really much more numerous—an insight that needs to be viewed as when interpreting benefits.